Join us for an exciting deep dive with Isaac Plutzer, a graduate researcher at Washington University in St. Louis, as he explores the mysterious ”dark kinome”—the hundreds of under-studied protein kinases with high potential for therapeutic discovery. In this interview, Isaac explains what kinases are and why a substantial fraction remain enigmatic, despite being critical regulators of cellular processes.

Isaac shares how his lab combines innovative proximity labeling and phosphoproteomics techniques with advanced mass spectrometry to systematically map the interactors, substrates, and signaling pathways of these mysterious enzymes. The conversation covers:

• What makes a kinase “dark” and why it matters for both basic biology and drug development

• How proximity labeling and mass spectrometry workflows (including TMT-based and DIA-based approaches) are expanding our map of protein interactions

• The unique challenges of complex data analysis—including imputation of missing values—and the evolving computational landscape

• Translational opportunities for new kinase-targeting drugs, and a look at the cutting edge of single-cell proteomics and automation

• Personal reflections on scientific discovery, experimental setbacks, and advice for young scientists entering the rapidly growing fields of proteomics and systems biology

Whether you’re passionate about biochemistry, analytical technology, or excited by scientific frontiers, this interview offers approachable insights into how next-generation tools are rewriting our understanding of the cell.

Listen in and be inspired by the thrill of the unknown, the promise of mass spectrometry, and the possibilities for the future of biomedical research!